Discussion
Which one of the following is the correct match between the molecular properties listed in Column I and the corresponding biochemical separation methods in Column II?
| Column I | Column II |
|---|---|
| P. Solubility | i. Reverse phase chromatography |
| Q. Ionic charge | ii. Ultracentrifugation |
| R. Polarity | iii. Salting out |
| S. Molecular size | iv. Isoelectric focusing |
| v. Gel electrophoresis |
Answer: C
The correct match is P-iii; Q-iv; R-i; S-ii.
- Solubility: salting out with ammonium sulfate precipitates proteins at different salt concentrations according to their solubility.
- Ionic charge: isoelectric focusing separates proteins by their isoelectric point in a pH gradient.
- Polarity: reverse phase chromatography retains molecules on a non-polar stationary phase according to their hydrophobicity.
- Molecular size: ultracentrifugation separates by sedimentation, which depends on size and mass.
Gel electrophoresis (v) also depends on size and charge, but only one option has the first three pairs right, and it uses ultracentrifugation for size.
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