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Cell lines are stored long term in liquid nitrogen chiefly because

  • A.the cells continue to divide slowly while stored
  • B.it is a good deal cheaper than a domestic freezer
  • C.the liquid nitrogen supplies nutrients to the cells
  • D.metabolic and chemical change effectively stop

Answer: D

At around minus 196 degrees Celsius no biological process proceeds at a measurable rate, so a stock can be kept for decades. An ordinary freezer allows slow deterioration to continue. Viability falls measurably over months in an ordinary minus eighty freezer.

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