Discussion
Consider the following statements about culture conditions.
1. Most mammalian cells are cultured at 37 degrees Celsius.
2. A carbon dioxide incubator maintains the pH of bicarbonate-buffered medium.
3. Cells grow equally well at room temperature.
Answer: D
The first two are correct. The third is wrong for mammalian cells, whose growth slows markedly below body temperature, though insect and fish lines are cultured cooler. The temperature follows the biology of the source organism.
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