Discussion
The major problem associated with the isolation of free cells and cell aggregates from organs is that of
Answer: A
Correct answer: releasing the cells from their supporting matrix
The cells of an organ are held in an extracellular matrix of collagen and other proteins, and the difficulty is freeing them from it without damaging them. Enzymes such as collagenase and trypsin, and chelators such as EDTA, are used for the purpose, and the balance is always between complete release and cell injury.
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