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What is the concentration of CO2 required for culturing animal cells?

  • A.2-5%
  • B.1-10%
  • C.10-15%
  • D.15-20%

Answer: B

Correct answer: 1-10%

Incubators are run at about 5% CO2, within a workable range of roughly 1-10%. The concentration is chosen to hold the bicarbonate buffer at physiological pH: too little and the medium turns alkaline, too much and it acidifies.

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