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Discussion

Taylor, Woods and Hughes labeled Vicia DNA by allowing new DNA synthesis in the presence of radioactive thymine. After DNA replication (S phase of the cell cycle), it was observed that

  • A.only one chromatid of a chromosome was labeled
  • B.both chromatids of a chromosome were labeled
  • C.both (a) and (b)
  • D.neither chromatid was labeled

Answer: B

Correct answer: both chromatids of a chromosome were labeled

After one round of replication in labelled thymine, every DNA molecule has one old (unlabelled) and one new (labelled) strand - so both sister chromatids carry label. That is exactly what semi-conservative replication predicts; a conservative mechanism would have left one chromatid entirely unlabelled. The asymmetry appears only in the second round.

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