Discussion
Which of the following proteins is NOT involved in opening a replication bubble?
Answer: D
Correct answer: ligase
Opening the bubble takes three kinds of protein: helicase to break the base pairs, single-strand binding protein to keep the separated strands from re-annealing, and topoisomerase to relieve the supercoiling that unwinding forces ahead of the fork. Ligase plays no part in opening - it comes later, sealing the nicks between Okazaki fragments.
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