Discussion
Near-Isogenic lines in plants can be developed by .......................
Answer: C
Near-isogenic lines are developed by the backcross method.
A donor line carrying a gene of interest is crossed to a recurrent parent, and the progeny are backcrossed to that parent for several generations while selecting for the gene. Each backcross restores more of the recurrent parent's genome, so the final lines differ from it essentially only around the selected gene.
Mass selection and the bulk method handle segregating populations without repeatedly returning to one parent, so they do not produce lines that differ at a single locus. Mutation breeding creates new variation rather than transferring a gene into an otherwise identical background.
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