Discussion
Which of the following are size exclusion methods ?
(a) Gel adsorption
(b) Gel filtration
(c) Gel diffusion
(d) Gel permeation
Choose the correct answer from the options given below :
Answer: D
The size exclusion methods are (b) and (d) only: gel filtration and gel permeation.
Size exclusion chromatography separates molecules by size as they pass through a porous gel. Small molecules enter the pores and take longer; large ones are excluded and elute first. When the mobile phase is aqueous (as for proteins) it is called gel filtration chromatography, and when it is an organic solvent (as for synthetic polymers) gel permeation chromatography.
Gel adsorption separates by adsorption onto a surface, not by size, and gel diffusion usually refers to immunodiffusion techniques such as Ouchterlony's, where antigens and antibodies diffuse through agar and precipitate.
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