Discussion
The commonly used light source in a fluorescence microscope is :
(a) Xenon arc lamp
(b) Mercury arc lamp
(c) Ruby laser
(d) Diode laser
Choose the correct answer from the options given below :
Answer: A
The common light sources are (a) and (b) only: the xenon arc lamp and the mercury arc lamp.
A fluorescence microscope needs an intense source of short-wavelength light to excite fluorescence. High-pressure mercury arc lamps (such as the HBO lamp) give strong emission lines in the ultraviolet and blue, and xenon arc lamps give a bright, fairly even spectrum across the visible range. These two have been the standard sources.
Lasers are used in specialised confocal systems rather than in the common fluorescence microscope, and a ruby laser, which emits deep red light, is unsuitable for exciting most fluorophores.
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