Discussion
One of the options mentioned is not a step involved in PCR technique :
Answer: D
Southern blotting is not a step in the polymerase chain reaction, option D. PCR copies a region of DNA by repeated cycles of denaturation, in which the strands are separated by heat, annealing, in which primers bind to the single strands, and extension, in which the polymerase adds nucleotides. Southern blotting is a separate method to detect a specific DNA sequence after electrophoresis and transfer to a membrane. Annealing (A) and extension (C) are steps of PCR, and the item printed as Deuaturation (B) is the denaturation step. A PCR run usually has about thirty cycles, and each cycle roughly doubles the amount of the target DNA, so that a few copies can be made into millions.
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