Discussion
Visualization of Esterase-D can be done using which of the following reagents ?
Answer: C
Esterase-D is visualised with 4-methyl umbelliferyl acetate. The enzyme splits this substrate to release the fluorescent 4-methyl umbelliferone, which shows as a bright band under ultraviolet light on the gel. Glycine-sodium carbonate solution is a buffer used to stop or enhance the fluorescence, glucose-1-phosphate is a substrate for phosphoglucomutase, and reduced glutathione is a substrate for glyoxalase I. Option C is correct.
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