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Which locus is used for determining both male and female gender in DNA fingerprinting ?

  • A.DYS 19
  • B.DYS 393
  • C.Amelogenin
  • D.Y-plex ladder

Answer: C

The locus used to determine both male and female gender in DNA fingerprinting is amelogenin. The amelogenin gene is on both X and Y chromosomes, in a form that gives a different fragment size on each, so a female gives a single X band and a male gives both X and Y bands. DYS19 and DYS393 are loci on the Y chromosome only, so they give a result only for a male, and a Y-plex ladder is a size marker, not a locus. Option C is correct.

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