Discussion
Jaffe test for urine is a reaction between
Answer: A
The Jaffe test is a reaction between creatinine and picric acid, option (A). In an alkaline solution the creatinine in urine or serum forms a red-orange coloured complex with picric acid, which is used for the colorimetric estimation of creatinine. Urea does not give this colour, so options with urea, (B) and (C), are wrong, and ammonium hydroxide is not the reagent, so (D) is wrong. The orange-red colour is read at about 500 nm and the method is simple, which is why it was in use for a long time in laboratories.
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