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The test to identify vaginal epithelial cells in a case of sexual assault is

  • A.Barberio's test
  • B.Florence's test
  • C.Lugol's iodine test
  • D.Precipitin test

Answer: C

The test is Lugol's iodine test.

For a sexual assault examination the swab is smeared on a slide, stained with Lugol's iodine, and examined under the microscope for the brown-stained flat cells.

Epithelial cells of the vagina are rich in glycogen, which stains mahogany brown with Lugol's iodine, so the cells can be recognised in a swab in a case of sexual assault.

Barberio's and Florence's tests are chemical tests for semen, using picric acid and iodine-potassium iodide, and the precipitin test identifies the species of origin of blood or other proteins. They do not identify vaginal epithelial cells.

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