Discussion
The most reliable test for detection of semen in the absence of spermatozoa is
Answer: C
The most reliable test for semen in the absence of spermatozoa is prostate specific antigen (p30).
PSA is made by the prostate and is present in seminal plasma even when the man is azoospermic or has had a vasectomy, and it is very specific to semen, so it is used as a confirmatory test.
Acid phosphatase and esterase are presumptive and can be found in other fluids, and the Florence test is a crystal test that is less reliable and gives false results.
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