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What does resolution between two peaks depend on?

  • A.Their separation alone, whatever their widths
  • B.Their heights relative to the baseline
  • C.The total time the analysis takes
  • D.Their separation and their widths together

Answer: D

Resolution is twice the difference in retention times divided by the sum of the two base widths, so both how far apart the peaks are and how broad they are matter equally. Height and analysis time do not enter. Two peaks well separated but very broad are no better resolved than two close sharp ones, which is why efficiency and selectivity must both be considered when a separation is being improved. The shared properties explain why these belong to one family, and the differences explain why more than one exists.

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