Discussion
Two peaks elute at \( 300 \) and \( 320 \ \mathrm{s} \) with widths of \( 8 \) and \( 9 \ \mathrm{s} \). Their resolution is about
Answer: A
Applying the standard expression:
\[ R_{s} = \frac{2(320-300)}{8+9} \approx 2.4 \]
giving about 2.4, comfortably above the 1.5 usually taken as baseline separation. Omitting the factor of two gives 1.2, which would wrongly suggest a marginal separation. A figure above 1.5 means the peaks may be integrated independently, which is the practical reason the threshold matters.
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