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What resolution is taken as baseline separation?

  • A.About 3.0
  • B.About 1.5
  • C.About 1.0
  • D.About 0.5

Answer: B

At 1.5 two equal peaks are separated essentially to the baseline and may be integrated independently, which is what quantitation requires. A resolution of one leaves a noticeable overlap, and three is comfortable but usually costs unnecessary analysis time. A threshold chosen because it is where a practical consequence changes, rather than for mathematical neatness, is worth knowing as a number. A single measurement chosen to respond to the one asymmetry beats several that respond to shared behaviour.

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