Discussion
Why is fast chromatography on the second column essential?
Answer: A
The modulation period sets a hard deadline: whatever the second column has not eluted when the next slice arrives is lost or overlapped. Detector speed is a real requirement that follows from this rather than causing it, and neither overheating nor gas consumption is limiting. A constraint imposed by one part of a system on another, rather than by any component's own capability, is what makes such designs so tightly coupled.
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