Discussion
What kind of sample most justifies two-dimensional chromatography?
Answer: B
Petroleum, essential oils and environmental extracts contain far more components than any single column's peak capacity can resolve, and only a second independent dimension helps. A pure compound, a single analyte and a lone impurity are all comfortably handled by one column. Reserving a complex technique for the problem that genuinely requires it is what keeps a laboratory productive, since the data handling alone is considerable.
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