Discussion
Why must extra-column volume be minimised in such a system?
Answer: C
A very efficient column produces peaks only a few microlitres wide, and a connection of comparable volume can undo much of the separation. Pump filling, detector saturation and equilibration are secondary. The better the column, the more the plumbing matters, which is why these instruments use finer tubing and smaller cells throughout rather than merely a stronger pump.
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