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Why does size exclusion have a limited peak capacity?

  • A.The detector cannot resolve close peaks
  • B.The eluent must be changed during the run
  • C.Everything elutes within one column volume
  • D.The pores are too narrow for large molecules

Answer: C

The first peak leaves at the interstitial volume and the last at the total permeation volume, so the entire chromatogram is squeezed into the difference between them. Pore size sets the range rather than the capacity, the detector is not the limit, and the eluent is held constant. A separation bounded at both ends by fixed volumes can never spread out, which is why the mode is used for molecular weight distributions rather than for resolving mixtures. Checking the units of each quantity catches a wrong formula before the arithmetic begins.

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