Discussion
What is hydrophilic interaction chromatography used for?
Answer: A
Polar analytes such as sugars and metabolites elute near the void in reversed phase, and a polar stationary phase with a mostly organic eluent retains them properly. Non-polar compounds, charged species and large molecules are handled by other modes. Reaching for a different mode when an analyte falls outside reversed phase's comfortable range is more effective than straining the dominant mode to cover everything. The shared properties explain why these belong to one family, and the differences explain why more than one exists.
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