Discussion
Which mode would suit separating a mixture of proteins by charge?
Answer: C
Ion exchange retains proteins by their net charge, which varies with pH, and elutes them with a salt gradient under conditions gentle enough to preserve activity. Reversed phase would denature them, size exclusion separates by size instead, and normal phase is unsuitable for aqueous samples. Choosing a mode means naming the property the separation is to exploit, and for proteins charge and size are the two usually available. A single measurement chosen to respond to the one asymmetry beats several that respond to shared behaviour.
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