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Discussion

Which of these best describes an operon?

  • A.A cluster of genes transcribed together from a single promoter
  • B.A single gene with several introns
  • C.A plasmid carrying resistance genes
  • D.A region of DNA that cannot be transcribed

Answer: B

Correct answer: A cluster of genes transcribed together from a single promoter

An operon is a group of adjacent genes transcribed together as one messenger RNA from a single promoter, so that functionally related proteins are produced in coordinated amounts. The arrangement is characteristic of prokaryotes and was described by Jacob and Monod in 1961. Its parts are the promoter, where RNA polymerase binds; the operator, where a regulatory protein binds; and the structural genes. A separate regulator gene encodes the protein that binds the operator. The lac operon is inducible: a repressor blocks transcription until lactose - through its isomer allolactose - binds the repressor and removes it, so the genes are expressed only when their substrate is present. The trp operon is repressible: it is transcribed until tryptophan accumulates and activates the repressor, switching it off. The two illustrate the general principle that catabolic pathways are induced by their substrate and anabolic ones repressed by their product.

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