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Nitrogen fixation by free-living aerobic bacteria such as Azotobacter is limited mainly by

  • A.The supply of available carbon in soil
  • B.The supply of atmospheric nitrogen reaching it
  • C.Soil temperature alone
  • D.The absence of legumes

Answer: A

Fixing nitrogen is expensive in energy, and a free-living organism must pay for it from soil organic carbon, which is usually scarce. A nodulated Rhizobium avoids the problem because the host supplies sugars directly, which is why symbiotic fixation contributes so much more.

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