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The expression of the following genes is essential for the lysogenic path of lambda phage when it infects E.coli :

  • A.N, Cro
  • B.CI, CII, CIII
  • C.P, O
  • D.att, xis, inf

Answer: B

The essential genes for lysogeny are CI, CII, CIII.

After lambda infects E. coli, the CII protein activates the promoters for the repressor gene cI and for the integrase gene; CIII protects CII from degradation by the host protease. The CI repressor then shuts down the lytic genes and maintains the prophage, so all three are needed for the lysogenic path.

Cro favours the lytic path, and N is an antiterminator used early in both paths. P and O are replication proteins of the lytic cycle, and xis is needed for excision of the prophage, not for establishing lysogeny.

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