Discussion
Why is a calibration curve preferred to a single standard?
Answer: C
Several standards across the range show whether the response really is a straight line through the origin, and where it begins to bend. A single point assumes linearity without testing it. It takes longer rather than less time, a blank is still needed, and stray light is not compensated. A calibration that tests its own assumption is worth the extra work, and it is what reveals the concentration range over which a result can be trusted. The quantity that varies and the quantity that does not are worth naming before any substitution is attempted.
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