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Why is ultraviolet detection common in liquid chromatography?

  • A.It measures the mass of each component
  • B.It requires no mobile phase transparency
  • C.Most analytes of interest absorb and the detector is simple
  • D.It responds equally to every compound eluting

Answer: C

A great many pharmaceutical and biological analytes contain conjugated systems, and a fixed-wavelength absorbance detector is cheap, robust and sensitive enough. It certainly does not respond equally to everything, which is a real limitation, it measures no masses, and the mobile phase must be transparent at the working wavelength. A detector that suits most of the analytes most of the time, at low cost, is often preferred to a universal one that costs ten times as much.

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