Discussion
In RIA; radiolabelling is done for :
Answer: A
In radioimmunoassay the Antigen is radiolabelled.
In the competitive RIA developed by Yalow and Berson, a fixed amount of radiolabelled antigen (for example insulin labelled with iodine-125) competes with the unlabelled antigen in the sample for a limited amount of antibody. The more antigen there is in the sample, the less labelled antigen is bound, and the concentration is read from a standard curve.
Labelling the antibody belongs to immunoradiometric assays (IRMA), a later variant, and labelled secondary antibodies belong to indirect techniques such as ELISA or immunoblotting with radioactive detection. The antigen-antibody complex is what is measured at the end, not what is labelled at the start.
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