Discussion
Match the test in Group I with its application in Group II
| Group I | Group II |
|---|---|
| P. Oakley-Fulthorpe test | 1. IgM detection |
| Q. Limulus amoebocyte lysate test | 2. Determining antigen-antibody specificity |
| R. Weil-Felix reaction test | 3. Endotoxin detection |
| S. Complement-fixation test | 4. Rickettsial infection diagnosis |
Answer: A
The match is P-2, Q-3, R-4, S-1.
- The Oakley-Fulthorpe test is a double immunodiffusion in tubes, used to examine antigen-antibody reactions and their specificity.
- The Limulus amoebocyte lysate test, which clots in the presence of lipopolysaccharide, detects bacterial endotoxin.
- The Weil-Felix reaction uses Proteus antigens that cross-react with rickettsial antibodies, so it is used to diagnose rickettsial infections.
- The complement-fixation test detects antibodies that fix complement; IgM fixes complement most efficiently, which is the pairing the key adopts.
Each of the other options misplaces at least one of the three unambiguous pairings: LAL with endotoxin, Weil-Felix with rickettsiae, and Oakley-Fulthorpe with antigen-antibody specificity.
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