Discussion
Monoclonal antibody production requires
Answer: C
Correct answer: both (a) and (b)
The hybridoma is made by fusing two cells, each supplying what the other lacks. The immunised mouse’s splenic lymphocytes provide the specific antibody but die in culture; the myeloma cells are immortal but make no useful antibody. Fused, the hybrid both secretes the chosen antibody and divides indefinitely, and selective medium kills the unfused partners.
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