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Fluorescent treponemal antibody test is an example of

  • A.indirect immunofluorescence
  • B.direct immunofluorescence
  • C.both (a) and (b)
  • D.none of these

Answer: A

Correct answer: indirect immunofluorescence

The FTA test fixes Treponema pallidum to a slide, adds the patient’s serum, and then detects any bound human antibody with a fluorescein-labelled anti-human globulin. Because the label is on the second antibody rather than on the first, it is an indirect method, and the extra layer amplifies the signal as well as allowing one labelled reagent to serve many tests.

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