Discussion
Amount of various immunoglobulin classes can be measured by
Answer: B
Correct answer: single diffusion in two dimensions
This is single radial immunodiffusion, the Mancini technique: antibody is incorporated into the gel and antigen is placed in a well, so only the antigen diffuses - hence single - and it spreads radially in two dimensions. The area of the ring of precipitate is proportional to the antigen concentration, which makes the method quantitative.
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