Discussion
For developing transgenic mustard variety DMH-11 Bar, Barnase and Barstar genes were obtained from :
Answer: A
The keyed answer is Bacillus amyloliquefaciens.
The hybrid mustard DMH-11, developed at Delhi University, uses the barnase-barstar system for hybrid seed production. Barnase, a ribonuclease expressed in the tapetum, makes the male line sterile, and barstar, its specific inhibitor, restores fertility in the hybrid. Both genes come from the soil bacterium Bacillus amyloliquefaciens.
The third gene, bar, which confers tolerance to the herbicide glufosinate and is used as a selectable marker, comes from Streptomyces hygroscopicus, which is not among the options; of those given, only B. amyloliquefaciens supplied genes. B. thuringiensis is the source of Bt insecticidal toxins, Agrobacterium tumefaciens is the vector used for gene transfer, and Erwinia uredovora supplied the crtI gene of Golden Rice.
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