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Why are samples sometimes run in ascending order of concentration?

  • A.To minimise carry-over into the lower readings
  • B.To let the flame stabilise gradually
  • C.To keep the calibration curve linear
  • D.To reduce the total volume aspirated

Answer: A

Running from low to high means any carry-over is from a weaker sample into a stronger one, where it matters proportionally less. Flame stability, linearity and volume are not affected by the order. Arranging a sequence so that its unavoidable errors fall where they do least harm is a cheap improvement available in many analytical procedures, and it costs only a moment's planning.

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