Discussion
What does a spike recovery experiment test?
Answer: A
Adding a known amount of analyte to a sample and measuring how much is found tests the whole procedure for losses and suppression together, and a recovery near a hundred per cent is the evidence a method works on that matrix. Linearity, noise and blanks are checked separately. Testing a method against a known answer, in the actual matrix, is the most convincing validation available short of a certified reference material. The quantity that varies and the quantity that does not are worth naming before any substitution is attempted.
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