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The cell wall of

  • A.gram-positive bacteria are thicker than gram-negative bacteria
  • B.gram-negative bacteria are thicker than gram-positive bacteria
  • C.both have same thickness but composition is different
  • D.none of these

Answer: A

Correct answer: gram-positive bacteria are thicker than gram-negative bacteria

The gram-positive wall is a thick multilayered sheet of peptidoglycan, some 20-80 nm; the gram-negative wall has only a thin layer of 2-7 nm, covered by an outer membrane. The thick layer retains the crystal violet-iodine complex when the cell is decolourised with alcohol, which is what makes the Gram stain work.

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