Discussion
The next to last step in peptidoglycan biosynthesis is
Answer: C
Correct answer: linking the sugar of the disaccharide-peptide unit to the growing peptidoglycan chain
The order of the last steps is: the subunit is carried across the membrane on bactoprenol, added to the growing glycan chain by transglycosylation, and only then cross-linked to a neighbouring strand by transpeptidation. So the next-to-last step is the attachment of the sugar, and cross-linking - the step penicillin blocks - is the last.
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