Discussion
Gram positive cells have a
Answer: B
Correct answer: multiple layer of peptidoglycan that helps to retain the crystal violet stain
The Gram stain works on wall thickness. The many layers of peptidoglycan in a gram-positive wall trap the crystal violet-iodine complex when alcohol is applied; the thin layer of a gram-negative cell does not, and its outer membrane is dissolved by the alcohol, so the cell decolourises and takes up the counterstain.
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