Discussion
Protoplasts can be generated from
Answer: A
Protoplasts can be generated from Streptococcus pneumoniae.
A protoplast is a cell whose wall has been removed completely, leaving only the plasma membrane. In Gram-positive bacteria the wall is a thick peptidoglycan layer with no outer membrane, so lysozyme (or growth with penicillin in an osmotically protected medium) strips it away entirely. S. pneumoniae is Gram-positive.
Brucella abortus, Pasteurella multocida and Shigella flexneri are Gram-negative. Removing their thin peptidoglycan leaves the outer membrane in place, so the product is a spheroplast, not a true protoplast.
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