Discussion
A gram-negative bacterium does not retain the crystal violet stain because
Answer: B
Correct answer: bacteria have thin peptidoglycan layer
A gram-negative cell has only a thin layer of peptidoglycan, and its outer membrane is dissolved by the alcohol used to decolourise. The crystal violet-iodine complex therefore washes straight out, and the cell takes up the safranin counterstain instead.
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