Discussion
A gram-positive bacterium retains the crystal violet stain because
Answer: D
Correct answer: bacteria have thick peptidoglycan layer
Retention is a matter of wall thickness. The many layers of peptidoglycan in a gram-positive wall dehydrate and shrink when alcohol is applied, trapping the crystal violet-iodine complex inside. The teichoic acids and the absence of a periplasm are genuine features of such cells, but they are not what holds the dye.
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