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Ziehl-Neelsen staining consist of

  • A.2 stages 
  • B.4 stages
  • C.3 stages 
  • D.5 stages

Answer: C

Correct answer: 3 stages

Ziehl-Neelsen has three steps: carbol fuchsin applied with heat, decolourisation with acid-alcohol, and a methylene blue counterstain. Acid-fast organisms such as mycobacteria keep the fuchsin, because their waxy mycolic acid wall resists the acid-alcohol.

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