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Discussion

Volutin granules are demonstrated by

  • A.Ozeshki’s staining
  • B.Burri’s technique
  • C.Loeffler’s technique
  • D.Morozov’s staining

Answer: C

Correct answer: Loeffler’s technique

Volutin (metachromatic) granules are polyphosphate stores, and they stain a different colour from the rest of the cell with methylene blue - the metachromasia that gives them their name. Loeffler’s alkaline methylene blue is the classic method, and Neisser’s stain is used for the same purpose in Corynebacterium diphtheriae.

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