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Dark ground microscopy is used for detection of

  • A.spirochetes
  • B.chlamydia
  • C.fungi
  • D.virus

Answer: A

Correct answer: spirochetes

Dark-ground (dark-field) microscopy lights the specimen obliquely so that only scattered light enters the objective, making a very thin unstained object shine against a black background. It is the standard way of seeing Treponema pallidum, which is too slender for ordinary bright-field work and stains poorly.

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