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Hemolytic serum for complement fixation reaction is obtained in the following manner

  • A.rabbits are immunized by suspension of sheep erythrocytes
  • B.sheep are immunized by suspension of rabbits erythrocytes 
  • C.guinea pig are immunized by suspension of sheep erythrocytes
  • D.rabbits are immunized by suspension of guinea pig erythrocytes

Answer: A

Correct answer: rabbits are immunized by suspension of sheep erythrocytes

Haemolytic serum is anti-sheep-red-cell antibody, and it is raised by injecting rabbits with sheep erythrocytes. The rabbit serum is then heated to destroy its own complement, so that the only complement in the final test is the one being measured.

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