Discussion
Which one of the following conjugations will result in formation of merodiploids?
Answer: C
Merodiploids result from an F′ donor × F− recipient cross.
An F′ (F-prime) plasmid is an F factor that has picked up a segment of chromosomal DNA. When it is transferred to an F− cell, the recipient gains a second copy of those chromosomal genes on the plasmid, so it is partially diploid, a merodiploid. This is the basis of complementation tests in bacteria.
An F+ × F− cross transfers only the F plasmid, with no chromosomal genes. In Hfr × F− crosses, chromosomal DNA does enter but is usually recombined into the recipient chromosome or lost, rather than kept as a stable second copy. An Hfr cell is not a recipient in the usual sense, so the last option is not a productive mating.
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