Discussion
Which one of the following redox couples has the highest tendency to donate electrons?
Answer: B
The strongest electron donor is the NAD+/NADH couple.
The tendency to donate electrons is measured by the standard reduction potential: the more negative it is, the more readily the reduced form gives up electrons. Approximate values at pH 7:
- NAD+/NADH: -0.32 V
- FAD/FADH2 (free): about -0.22 V
- Pyruvate/lactate: -0.19 V
- Fumarate/succinate: +0.03 V
NADH has the most negative potential and so the highest tendency to donate electrons, which is why it feeds the respiratory chain at its start. Succinate is the weakest donor of the four.
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